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pc3 human prostate carcinoma tumour cells  (ATCC)


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    ATCC pc3 human prostate carcinoma tumour cells
    Fig. 7. A. Western blotting analysis of the effect of a-toxin on activation of NF-kB. Both A549 and <t>PC3</t> cells were grown to 70% confluence in tissue culture plates, treated with a-toxin (0.1 mg ml-1), and then incubated for 2, 4 and 6 h at 5% CO2, 37°C. The whole cell lysates were prepared from the treated cells and control. The same amounts of proteins were loaded on a 12% SDS-PAGE and blotted with anti-I-kB antibody. B. The impact of various inhibitors of NF-kB on cytotoxicity of a-toxin. A549 cells were grown to 70% confluence in 96 well plates, treated with lactacystin (2 mg ml-1) and PDTC (1 mg ml-1), respectively, for 1 h before adding a-toxin (1 mg ml-1), and then incubated for 16 h at 5% CO2, 37°C. Cell viability was measured after treatment and is expressed as an average of at least three experiments standard deviation. The symbol ‘*’ represents P < 0.05.
    Pc3 Human Prostate Carcinoma Tumour Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 14529 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pc3+human+prostate+carcinoma+tumour+cells/PC-3/pm17359518-182-9-15
    Average 99 stars, based on 14529 article reviews
    pc3 human prostate carcinoma tumour cells - by Bioz Stars, 2026-09
    99/100 stars

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    1) Product Images from "Involvement of alpha5beta1-integrin and TNF-alpha in Staphylococcus aureus alpha-toxin-induced death of epithelial cells."

    Article Title: Involvement of alpha5beta1-integrin and TNF-alpha in Staphylococcus aureus alpha-toxin-induced death of epithelial cells.

    Journal: Cellular microbiology

    doi: 10.1111/j.1462-5822.2007.00917.x

    Fig. 7. A. Western blotting analysis of the effect of a-toxin on activation of NF-kB. Both A549 and PC3 cells were grown to 70% confluence in tissue culture plates, treated with a-toxin (0.1 mg ml-1), and then incubated for 2, 4 and 6 h at 5% CO2, 37°C. The whole cell lysates were prepared from the treated cells and control. The same amounts of proteins were loaded on a 12% SDS-PAGE and blotted with anti-I-kB antibody. B. The impact of various inhibitors of NF-kB on cytotoxicity of a-toxin. A549 cells were grown to 70% confluence in 96 well plates, treated with lactacystin (2 mg ml-1) and PDTC (1 mg ml-1), respectively, for 1 h before adding a-toxin (1 mg ml-1), and then incubated for 16 h at 5% CO2, 37°C. Cell viability was measured after treatment and is expressed as an average of at least three experiments standard deviation. The symbol ‘*’ represents P < 0.05.
    Figure Legend Snippet: Fig. 7. A. Western blotting analysis of the effect of a-toxin on activation of NF-kB. Both A549 and PC3 cells were grown to 70% confluence in tissue culture plates, treated with a-toxin (0.1 mg ml-1), and then incubated for 2, 4 and 6 h at 5% CO2, 37°C. The whole cell lysates were prepared from the treated cells and control. The same amounts of proteins were loaded on a 12% SDS-PAGE and blotted with anti-I-kB antibody. B. The impact of various inhibitors of NF-kB on cytotoxicity of a-toxin. A549 cells were grown to 70% confluence in 96 well plates, treated with lactacystin (2 mg ml-1) and PDTC (1 mg ml-1), respectively, for 1 h before adding a-toxin (1 mg ml-1), and then incubated for 16 h at 5% CO2, 37°C. Cell viability was measured after treatment and is expressed as an average of at least three experiments standard deviation. The symbol ‘*’ represents P < 0.05.

    Techniques Used: Western Blot, Activation Assay, Incubation, Control, SDS Page, Standard Deviation



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    pc3 human prostate carcinoma tumour cells  (ATCC)
    99
    ATCC pc3 human prostate carcinoma tumour cells
    Fig. 7. A. Western blotting analysis of the effect of a-toxin on activation of NF-kB. Both A549 and <t>PC3</t> cells were grown to 70% confluence in tissue culture plates, treated with a-toxin (0.1 mg ml-1), and then incubated for 2, 4 and 6 h at 5% CO2, 37°C. The whole cell lysates were prepared from the treated cells and control. The same amounts of proteins were loaded on a 12% SDS-PAGE and blotted with anti-I-kB antibody. B. The impact of various inhibitors of NF-kB on cytotoxicity of a-toxin. A549 cells were grown to 70% confluence in 96 well plates, treated with lactacystin (2 mg ml-1) and PDTC (1 mg ml-1), respectively, for 1 h before adding a-toxin (1 mg ml-1), and then incubated for 16 h at 5% CO2, 37°C. Cell viability was measured after treatment and is expressed as an average of at least three experiments standard deviation. The symbol ‘*’ represents P < 0.05.
    Pc3 Human Prostate Carcinoma Tumour Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pc3+human+prostate+carcinoma+tumour+cells/PC-3/pm17359518-182-9-15
    Average 99 stars, based on 1 article reviews
    pc3 human prostate carcinoma tumour cells - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier

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    Fig. 7. A. Western blotting analysis of the effect of a-toxin on activation of NF-kB. Both A549 and PC3 cells were grown to 70% confluence in tissue culture plates, treated with a-toxin (0.1 mg ml-1), and then incubated for 2, 4 and 6 h at 5% CO2, 37°C. The whole cell lysates were prepared from the treated cells and control. The same amounts of proteins were loaded on a 12% SDS-PAGE and blotted with anti-I-kB antibody. B. The impact of various inhibitors of NF-kB on cytotoxicity of a-toxin. A549 cells were grown to 70% confluence in 96 well plates, treated with lactacystin (2 mg ml-1) and PDTC (1 mg ml-1), respectively, for 1 h before adding a-toxin (1 mg ml-1), and then incubated for 16 h at 5% CO2, 37°C. Cell viability was measured after treatment and is expressed as an average of at least three experiments standard deviation. The symbol ‘*’ represents P < 0.05.

    Journal: Cellular microbiology

    Article Title: Involvement of alpha5beta1-integrin and TNF-alpha in Staphylococcus aureus alpha-toxin-induced death of epithelial cells.

    doi: 10.1111/j.1462-5822.2007.00917.x

    Figure Lengend Snippet: Fig. 7. A. Western blotting analysis of the effect of a-toxin on activation of NF-kB. Both A549 and PC3 cells were grown to 70% confluence in tissue culture plates, treated with a-toxin (0.1 mg ml-1), and then incubated for 2, 4 and 6 h at 5% CO2, 37°C. The whole cell lysates were prepared from the treated cells and control. The same amounts of proteins were loaded on a 12% SDS-PAGE and blotted with anti-I-kB antibody. B. The impact of various inhibitors of NF-kB on cytotoxicity of a-toxin. A549 cells were grown to 70% confluence in 96 well plates, treated with lactacystin (2 mg ml-1) and PDTC (1 mg ml-1), respectively, for 1 h before adding a-toxin (1 mg ml-1), and then incubated for 16 h at 5% CO2, 37°C. Cell viability was measured after treatment and is expressed as an average of at least three experiments standard deviation. The symbol ‘*’ represents P < 0.05.

    Article Snippet: A549 human lung epithelial cells (ATCC CCL 185) and PC3 human prostate carcinoma tumour cells (ATCC CRL-1435TM) were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS; Invitrogen, CA).

    Techniques: Western Blot, Activation Assay, Incubation, Control, SDS Page, Standard Deviation